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Comparison of DNA repair protein expression and activities

These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. Rabbit polyclonal Ku70 + Ku80 antibody. Validated in WB, IHC and tested in Human. Cited in 4 publication(s).

853 m avv. HUVUDNIVÅ 1078 GEMENSAMT. KC100 1078 m avv och KU70. 740 m avv.

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Although Ku80 was recently 2016-07-01 · Human Ku70/80 was added to a final concentration of 20 ng/μl. Compound L was added as solution in DMSO (final concentration DMSO in the EMSA reaction mixture was 5%). This reaction containing Ku70/80 and Compound L was then allowed to pre-incubate for 5 min at 37 °C. The Ku70/80 heterodimer is a major player in non-homologous end joining and the repair of DNA double-strand breaks.

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Classification: RNA BINDING PROTEIN; Organism(s): The Ku70/80 heterodimer is a key-player in the NHEJ pathway and binds to DNA termini with high affinity, where it helps to protect DNA ends from degradation 6 Oct 2020 Knockdown of Ku70 or Ku80 in naïve mice elicited mitochondrial collapse or ER stress, leading to bronchial epithelial cell apoptosis and 25 Jun 2020 During DSB repair by NHEJ, Ku70 plays a key role in conjunction with Ku80 to form the Ku70–Ku80 heterodimer. This complex has a Provided herein are methods for identifying and treating subjects having conditions involving aberrant Ku70/80 activity. In particular, the invention relates to Inhibition of the Ku DNA end-binding activity by transfection with the C-terminal Ku80 expression gene suppressed cell proliferation. Ku70 or Ku80 overexpression Ku70 — белок, в человеческом организме кодируемый геном XRCC6. Содержание. 1 Функция; 2 Старение; 3 Номенклатура; 4 Взаимодействия; 5 Примечания.

However, the precise biological consequence, the biochemical nature of these interactions and the role of DNA-PK in telomere maintenance remain unknown. 2012-2-10 · Human Ku70/80 protein blocks exonuclease 1-mediated DNA resection in the presence of human Mre11 or Mre11/Rad50 protein complex. Sun J(1), Lee KJ, Davis AJ, Chen DJ. Author information: (1)Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA. 2020-10-4 · The Ku70 was similarly decreased (by 2.1-, 1.5- and 1.7-fold, respectively) in Ku80+/- cells. sites within the imprinting control center of the H19 and Igf2 genes bind Ku70/80 in a sequence-specific manner and with higher affinity than previously reported binding sites The Ku70/80 heterodimer binds to DNA ends and attracts other proteins involved in the non-homologous end-joining (NHEJ) pathway of DNA double-strand break repair. We developed a novel assay to measure DNA binding and release kinetics using differences in Förster resonance energy transfer (FRET) of the ECFP-Ku70/EYFP-Ku80 heterodimer in soluble and DNA end bound states. Double strand break (DSB) recognition is the first step in the DSB damage response and involves activation of ataxia telangiectasia-mutated (ATM) and phosphorylation of targets such as p53 to trigger cell cycle arrest, DNA repair, or apoptosis.
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They are encoded by the Xrcc5 and Xrcc6 genes, respectively, and are highly abundant in both prokaryotes and eukaryotes. The stability of these proteins depends on each other. The nuclear DNA repair protein Ku70/80 is a tumor-associated antigen displaying rapid receptor mediated endocytosis. / Fransson, Johan; Borrebaeck, Carl..

Author links open overlay panel Takuya Abe a Masamichi Ishiai b Yoshifumi Hosono a Akari Yoshimura a Shusuke Tada a Noritaka Adachi c Hideki Koyama c Minoru Takata b Shunichi Takeda d Takemi Enomoto a e Masayuki Seki a. The yeast homologue of Ku70/80, yKu70/80, fails to bind IP 6, indicating that the function of IP 6 in non‐homologous end‐joining, like that of DNA‐PK cs, is unique to the mammalian end‐joining process. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 + Ku80 antibody (ab53126) ab53126 at 1/50 dilution staining Ku70 + Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue in the absence and … Our previous studies indicated that the Werner syndrome protein (WRN) interacts with Ku, a heterodimeric factor of 70- and 80-kDa subunits implicated in the repair of double strand DNA breaks.
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Immunogen corresponding to synthetic peptide. 2013-05-01 · When both Ku70/80 and RECQ1 were incubated with fork duplex in the presence of ATPγS, we observed a super-shifted species that is distinct from those obtained with binding of the fork DNA with Ku70/80 or RECQ1 alone (Fig.

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2005-4-11 · Ku70/80 associates with telomeric DNA and binds to TRF1 and TRF2 (14,15). Moreover, Ku has been reported to associate with hTERT and telomerase activity . However, the precise biological consequence, the biochemical nature of these interactions and the role of DNA-PK in telomere maintenance remain unknown.